Animal Tissue Culture

Animal cell culture involves isolation of cells from a tissue before establishing a culture in a suitable artificial environment. Initial isolation of the cells from the tissues can be achieved by disaggregation using enzymatic or mechanical methods. In CytoGene we provide training from media preparation to observation and analysis of growth pattern under inverted microscope. we provide 30 days program to provided complete hands on training experiments.

Training Batches in 2020

Batches: 1st, 10th & 20th of every Month

No of Candidate: 10 / Batch

Date of Notification: 01 May 2020

Start of Registration: 01 May 2020

Registration Process: Online & Offline

Training @ CytoGene

Duration: 1 Month


Registration Fee: ₹ 1,000/-

(Fee ₹847.5 + GST ₹152.5)

Training Fee: ₹ 10,000/-

(Fee ₹8475 + GST ₹1525)

ACC01    Culture of egg embryo & Morphological study

  • Preparation of Media

  • Preparation of Sera

  • Primary culture of chick embryo fibroblast

  • Sub culture of primary culture

  • Study of developmental stages & growth pattern

ACC02    Possessing and Maintenance of Cell line

  • Primary culture of fish epithelial cell

  • Sub culture of primary culture of fish epithelial cell

  • Passaging of cultures

  • Preservation of cells

  • Observation & growth pattern under inverted microscope

ACC03    Cytotoxic Assessment (MTT Assay)

  • Growth of target cell line

  • Sample preparation (Extract)

  • Application of Extract on cell line

  • Observation of growth pattern under Inverted Microscope

  • Quantitative assay by ELISA

ACC04    Study of Multidrug effect on Cell line

  • Selection and preparation of Multiple drug combination with ratio

  • selection and culture of cell line

  • Application of Multidrug on culture

  • Continuous observation of growth pattern after every time interval

  • Quantitative study after every time interval

  • Analysis of observations & datas

ACC05    Study of Cytotoxic effect of food sample

  • Food additive cytotoxicity determination (Antioxidant BHA, BDT)

  • Cytotoxicity of Soft drinks in Human Hepatoma Hep G2 cells

  • Sample preparation and extraction of heterocyclic aromatic amines

  • HepG2 cells: culture and maintenance

  • Single cell gel electrophoresis assay/ Comet assay



Reporting Office : 

CSC (B) – 301, 2nd floor,

Old Complex, 

Sahara States, Jankipuram,

Lucknow - 226021

Laboratory : 

K-51, Agro Park,
UPSIDC Industrial Area
Kursi Road (Lucknow)

Dist-Barabanki – 225001

Tele : 



Email :

CytoGene Research & Development

Disclaimer and Copyright Information


Information in this website is correct at the time of design, but may change from time to time. In particular CytoGene reserves the right to change content and/or method of assessment, to change or alter fees of any Services, to withdraw any experiment or program that it offers, to impose limitations on enrolment in any unit or program, and/or to vary arrangements for any program. CytoGene will not be liable to you or to any other person for any loss or damage (including direct, consequential or economic loss or damage) however caused and whether by negligence or otherwise that may result directly or indirectly from the use of this website. Copyright Lucknow of CytoGene 2018.  Apart from any fair for the purposes of private study, research, criticism or review, as permitted under the Copyright Act. no part may be reproduced by any process without written permission. Enquiries should be directed to the CytoGene Office at Lucknow on 77-0309-2222. Published by CytoGene Research & Development LLP.

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