ANIMAL CELL CULTURE
Animal Tissue Culture
Animal cell culture involves isolation of cells from a tissue before establishing a culture in a suitable artificial environment. Initial isolation of the cells from the tissues can be achieved by disaggregation using enzymatic or mechanical methods. In CytoGene we provide training from media preparation to observation and analysis of growth pattern under inverted microscope. we provide 30 days program to provided complete hands on training experiments.
Training Batches in 2020
Batches: 1st, 10th & 20th of every Month
No of Candidate: 10 / Batch
Date of Notification: 01 May 2020
Start of Registration: 01 May 2020
Registration Process: Online & Offline
Training @ CytoGene
Duration: 1 Month
Registration Fee: ₹ 1,000/-
(Fee ₹847.5 + GST ₹152.5)
Training Fee: ₹ 10,000/-
(Fee ₹8475 + GST ₹1525)
ACC01 Culture of egg embryo & Morphological study
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Preparation of Media
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Preparation of Sera
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Primary culture of chick embryo fibroblast
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Sub culture of primary culture
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Study of developmental stages & growth pattern
ACC02 Possessing and Maintenance of Cell line
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Primary culture of fish epithelial cell
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Sub culture of primary culture of fish epithelial cell
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Passaging of cultures
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Preservation of cells
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Observation & growth pattern under inverted microscope
ACC03 Cytotoxic Assessment (MTT Assay)
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Growth of target cell line
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Sample preparation (Extract)
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Application of Extract on cell line
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Observation of growth pattern under Inverted Microscope
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Quantitative assay by ELISA
ACC04 Study of Multidrug effect on Cell line
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Selection and preparation of Multiple drug combination with ratio
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selection and culture of cell line
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Application of Multidrug on culture
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Continuous observation of growth pattern after every time interval
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Quantitative study after every time interval
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Analysis of observations & datas
ACC05 Study of Cytotoxic effect of food sample
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Food additive cytotoxicity determination (Antioxidant BHA, BDT)
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Cytotoxicity of Soft drinks in Human Hepatoma Hep G2 cells
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Sample preparation and extraction of heterocyclic aromatic amines
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HepG2 cells: culture and maintenance
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Single cell gel electrophoresis assay/ Comet assay
ANIMAL CELL CULTURE
(ACC)