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Molecular Biology

Molecular biology is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated. In CytoGene, we offer several modules of training in Molecular Biology. The duration of the programs offered for training ranges from 15 & 30 days. Modules are selected by the students on their choice.

Discontinued...
(Currently we are not providing training in this module)

30 DAYS

Training Module

FEE

Registration Fee: ₹ 500/-

Training Fee: ₹ 8,000/-

ADVANCE MOLECULAR BIOLOGY (MOL)

  • ​Good laboratory practices

  • Demonstration of all Instruments

  • Chemical calculation & Reagent Preparation

​

​Extractions and Purifications

  • DNA isolation from bacteria

  • Agarose Gel Electrophoresis

  • Plasmid DNA isolation & Electrophoresis

  • DNA isolation from plant leaves & Electrophoresis

  • Estimation of Nucleotide by UV – Vis Spectrophotometer

  • RNA Isolation from Plant

  • Formaldehyde Gel Electrophoresis

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Amplification of Gene by PCR

  • Selection of Gene of Interest

  • Primer Designing

  • Preparation of Master mix

  • Amplification of Target Gene by PCR

  • Setup of Multiplex PCR

  • Analysis of Multiplex PCR on Agarose Gel Electrophoresis

​​

Molecular Identification of Species

  • Genomic DNA Isolation (Bacteria)

  • Agarose Gel Electrophoresis

  • PCR Amplification using Universal Primer

  • DNA Sequencing (Theory only)

  • Bioinformatic Analysis.

  • Conversion of raw data to sequence form by bio edit

  • Combining of forward sequence with reverse sequence

  • Blast & Phylogenetic Analysis of 16S/18S rRNA Sequence

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Application of Molecular Marker

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • PCR Amplification using RAPD Primers

  • RAPD Analysis by software

  • Restriction Digestion for RFLP and Agarose Gel Electrophoresis

  • Result Interpretation of RFLP and their Application

15 DAYS

Training Module

FEE

Registration Fee: ₹ 500/-

Training Fee: ₹ 5,000/-

BASIC MOLECULAR BIOLOGY (BMO)

  • Good laboratory practices

  • Demonstration of all Instruments

  • Chemical calculation & Reagent Preparation

  • DNA isolation from bacteria

  • Agarose Gel Electrophoresis

  • Plasmid DNA isolation & Electrophoresis

  • DNA isolation from plant leaves & Electrophoresis

  • Estimation of Nucleotide by UV – Vis Spectrophotometer

  • RNA Isolation from Plant

  • Formaldehyde Gel Electrophoresis

  • Selection of Gene of Interest

  • Primer Designing

  • Preparation of Master mix

  • Amplification of Target Gene by PCR

  • Analysis of PCR product on Agarose Gel Electrophoresis

  • Genomic DNA Isolation from Blood

  • Restriction Digestion for RFLP and Agarose Gel Electrophoresis

  • Result Interpretation of RFLP and their Application

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