Customised Biotech Training

Tired with old pattern of Training


Okey! Now you decide...

Your Syllabus!

Your Joining Date!

Your Batchmates!

Your Duration!

Your Time Schedule!

CytoGene, Lucknow brings Customised Training program, for the candidate interested to choose any of their interest of techniques. There will be requirement of minimum 5 and maximum 10 candidate to complete a batch. It has an aim to train on advance techniques without going through the basic experiments. This techniques will provide a signature training for the students of PhD, Research Scholars & Teachers.

Customised Training 2020

No of Minimum Candidate Required: 5


     10 Day's (Any two techniques)

     15 Day's (Any three techniques)

     30 Day's (Any five techniques)

Fee per Candidate:

      Registration Fee:

      ₹500 (Fee ₹424 + GST ₹76)

     Training Fee:

     10 Day's : ₹3,000 (Fee ₹2,542 + GST ₹458

     15 Day's : ₹4,000 (Fee ₹3,390 + GST ₹610) 

     30 Day's : ₹6,000 (Fee ₹5,085 + GST ₹915) 

Date of Joining: Opted by Candidate (Through out the year)


MCR01    Antimicrobial Activity

  • Antimicrobial by Agar Diffusion Method

  • Antimicrobial by Disc Diffusion Method

  • Minimum Bactericidal Concentration (MBC)

  • Minimum Inhibitory Concentration (MIC) assay

MCR02    Antagonistic Activity

  • Preparation of Broth of cultures

  • Agar Plug Diffusion Method

  • Cross Streak Method

  • Comparison of Growth Kinetics

MCR03    Production of Bio-surfactants & its characterisation

  • Screening of PGPR Bacteria

  • Production of Bio-surfactant on fermentation media

  • Purification of Bio-surfactants

  • Characterization by FTIR & Analysis of Results

MCR04    Identification of Microbes by Multiplex PCR

  • Culture of sample on specific media

  • Purification by streak

  • DNA Isolation of microbe

  • Setup of multiplex PCR

  • Analysis of results

MCR05    Molecular Characterisation of Microbes

  • DNA Isolation from bacteria

  • Agarose gel electrophoresis

  • PCR Amplification of 16S rRNA Gene

  • Sequencing (Lecture only)

  • Bioinformatic Analysis


MOL01    DNA Barcoding

  • Genomic DNA from bacteria, Agarose Gel Electrophoresis & Quantification

  • PCR Amplification of 16S rRNA Gene against DNA from Bacteria

  • Sequencing (Lecture only)

  • Sequence Analysis by Bioinformatics

MOL02    Restriction Fragment Length Polymorphism (RFLP)

  • Genomic DNA From Blood, Agarose Gel Electrophoresis & Quantification

  • Set up of PCR for RFLP against DNA from blood

  • Set up of Restriction Digestion Reaction

  • RFLP Analysis by software

MOL03    Random Amplification of Polymorphic DNA (RAPD)

  • Genomic DNA From Plant Leaf, Agarose Gel Electrophoresis & Quantification

  • Set up of PCR for RAPD against DNA from plant leaf,

  • RAPD Analysis by software

MOL04    RNA Analysis

  • RNA Extraction from blood

  • Formaldehyde Gel Electrophoresis & Quantification

MOL05    Karyotyping

  • Synchronizing cells

  • Releasing the chromosomes from their cells

  • Staining of chromosomes

  • Karyotyping analysis


BCH01    Nutritional Quality Evaluation of Food Materials

  • Moisture

  • Fat

  • Carbohydrate

  • Mineral

  • Protein by Kjeldahl Method

  • Ash Content

BCH02    Phytochemical Activity

  • Glycoside Test

  • Alkaloid Test

  • Terpenoids Test

  • Saponin Test

BCH03    High Performance Liquid chromatography (HPLC)

  • Thin Layer Chromatography on Silica Gel

  • Column Chromatography

  • HPLC parts and its functioning

  • Set up of HPLC

BCH04    SDS PAGE & Western blotting

  • Set up of SDS PAGE Staining, de-staining & Analysis

  • Set up of Native PAGE Staining, de-staining & Analysis

  • Set up of Western Blotting & Transfer

  • Application of primary and secondary antibody & Analysis of Results

BCH05    Enzyme Kinetics, IC Value & Immobilisation

  • Enzyme characterization

  • Enzyme assay

  • Enzyme Activity

  • Enzyme Kinetics

  • Dialysis of Enzyme / Protein

  • IC 50 Calculation


  • Moisture Content Determination

  • Lipid / Fat Content determination (Soxhlet method for solid samples; Gerber method For Dairy products).

  • Protein Content Determination (Kjeldahl Method; Lowry’s Assay)

  • Carbohydrate Content determination (Anthrone method)

FDT02    Food Microbiology and Microbial Toxicity

  • Aerobic / Anaerobic Mesophilic Count

  • Total Coliform Bacteria (Thermotolerant)

  • Total Yeast and Mould Count

  • Food Nutrition and Microbes – Antagonistic activity

  • Broth  dilution assay (Antimicrobial Assay)

  • Sample preparation of food product 

  • Parts & its Functions of HPLC

  • Calibration, Validation and Estimation of LOD, LOQ, RSD.

  • Sugar content profiling through HPLC.

  • Vitamin content profiling through HPLC.

FDT03    Application of HPLC in Food Technology

FDT04    Application of ELISA in Food samples

  • Extraction of Deoxynivalenol (DON) from cereals (Soxhlet method)

  • Purification through Column Chromatography (Silica Gel H)

  • Quantitative determination (Subjective interaction, LOD, LOQ)

  • Endotoxin Determination by ELISA.

FDT05    Cytotoxic Study of Food Sample

  • Food additive cytotoxicity determination (Antioxidant BHA, BDT)

  • Cytotoxicity of Soft drinks in Human Hepatoma Hep G2 cells

  • Sample preparation and extraction of heterocyclic aromatic amines

  • HepG2 cells: culture and maintenance

  • Single cell gel electrophoresis assay/ Comet assay

FDT01    Proximate Analysis

Reporting Office : 

CSC (B) – 301, 2nd floor,

Old Complex, 

Sahara States, Jankipuram,

Lucknow - 226021

Laboratory : 

K-51, Agro Park,
UPSIDC Industrial Area
Kursi Road (Lucknow)

Dist-Barabanki – 225001

Tele : 



Email :

CytoGene Research & Development

Disclaimer and Copyright Information


Information in this website is correct at the time of design, but may change from time to time. In particular CytoGene reserves the right to change content and/or method of assessment, to change or alter fees of any Services, to withdraw any experiment or program that it offers, to impose limitations on enrolment in any unit or program, and/or to vary arrangements for any program. CytoGene will not be liable to you or to any other person for any loss or damage (including direct, consequential or economic loss or damage) however caused and whether by negligence or otherwise that may result directly or indirectly from the use of this website. Copyright Lucknow of CytoGene 2018.  Apart from any fair for the purposes of private study, research, criticism or review, as permitted under the Copyright Act. no part may be reproduced by any process without written permission. Enquiries should be directed to the CytoGene Office at Lucknow on 77-0309-2222. Published by CytoGene Research & Development LLP.

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