Recombinant DNA Technology

Recombinant DNA Technology is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. r-DNA Technology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of cloning and gene transfer. In CytoGene, we offer several modules of training in r-DNA Technology. The duration of the programs offered for training ranges from 15 & 30 days. Modules are selected by the students on their choice.

Training Batches in 2022

This module is not Available in this Summer (2022)

The following modules are available for Summer training

Biotechnology

Food Analysis & Quality Control

Immunology

30 DAYS

Training Module

FEE

Registration Fee: ₹ 500/-

(Fee ₹424 + GST ₹76)

Training Fee: ₹ 8,000/-

(Fee ₹6,780 + GST ₹1,220)

REGISTRATION OFFER

Register on or before 15th April and get 

10% concession on training fee

or

Register on or before 30th April and get

05% concession on training fee

15 DAYS

Training Module

FEE

Registration Fee: ₹ 500/-

(Fee ₹424 + GST ₹76)

Training Fee: ₹ 5,000/-

(Fee ₹4,237 + GST ₹763)

RECOMBINANT DNA TECHNOLOGY (RDT)

  • Good laboratory practices

  • Demonstration of all Instruments

  • Chemical calculation & Reagent Preparation

 

General Bioinformatics

  • Selection Selection of gene from database

  • Selection of Vector

  • Primer Designing of Gene of Interest

Gene Cloning by traditional Method

  • Genomic DNA Isolation

  • Agarose Gel electrophoresis

  • Quantification by UV- Vis Spectrophotometer

  • PCR Amplification of Gene of Interest

  • Restriction Digestion

  • Ligation

  • Preparation of competent cells

  • Transformation by Heat shock method

  • Screening by Antibiotic Resistant gene

 

Gene cloning by CRISPR

  • Selection of gene from database

  • Designing of spacer sequence by E-CRISPR tool

  • Construction of crRNA-Cas9 plasmid (Digestion)

  • Construction of crRNA-Cas9 plasmid (Ligation)

  • Preparation of competent cells of E. coli DH5-α

  • Transformation of E. coli DH5-α 

  • Screening of Transformants

  • Plasmid DNA Isolation from E.coli DH5 Alpha

  • Agarose Gel Electrophoresis of Isolated plasmid

  • Competent Cell preparation

  • Transformation of S. Cerevisiae with crRNA-Cas9 Plasmid

  • Screening on media for selection of Transformants

  • Genomic DNA isolation from positive clones

  • Interpretation of gene deletion by PCR amplification

  • Observation of result on Agarose Gel

GENE CLONING BY CRISPR

(GCC)

  • Good laboratory practices

  • Demonstration of all Instruments

  • Chemical calculation & Reagent Preparation

  • Selection of gene from database

  • Designing of spacer sequence by E-CRISPR tool

  • Construction of crRNA-Cas9 plasmid (Digestion)

  • Construction of crRNA-Cas9 plasmid (Ligation)

  • Preparation of competent cells of E. coli DH5-α

  • Transformation of E. coli DH5-α 

  • Screening of Transformants

  • Plasmid DNA Isolation from E. coli DH5 Alpha

  • Agarose Gel Electrophoresis of Isolated plasmid

  • Competent Cell preparation

  • Transformation of S. Cerevisiae with crRNA-Cas9 Plasmid

  • Screening on media for selection of Transformants

  • Genomic DNA isolation from positive clones

  • Interpretation of gene deletion by PCR amplification

  • Observation of result on Agarose Gel