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Hands-on-Training

on

"Biotechnological Instrumentation"

CytoGene offers training in Biotechnology. Student can avail Hands-on-training of basic techniques at a nominal charges. The duration of the programs offered for 7, 15 & days havin techniques of Microbiology,

Molecular Biology, Biochemistry & Enzyme Technology.

TRAINING  INCLUDES

1. CHEMICAL CALCULATION AND REAGENT PREPARATION

This includes a thorough summarisation of fundamentals of calculation required during training. This helps the candidate to gain basic knowledge on Molarity, Normality etc.

3. LABORATORY WORK AND PERFORMING EXPERIMENTS

Students has to perform one by one all the listed experiments in any chooses modules, supervisor will instruct throughout training. The laboratory work has to be performed by students only on individual basis. Supervisor will not perform any experiments on behalf of candidate. 

5. REVIEW OF COMPLETE WORK AND Q&A SESSION

A review of the complete work will be done by supervisor to ensure all the work done and findings are grasped by the candidates. There will be a question and answer session also to clear all the doubts and queries (if any).

2.  DISCUSSION ON GOOD LABORATORY PRACTICES

Before proceeding for any laboratory work  the adaptation of good laboratory practices is needed to ensure better handling of instruments and reagents. We compel to adapt the GLP inside the lab.

4. DAILY RECORD OF WORK DONE IN LABORATORY MANUAL

All the results obtained during the work must be managed in very organised form with complete analysis. A manual will be provided by CytoGene at the beginning of training itself. The daily output of experiments have to be filled in this manual.

6. REPORT PREPARATION AND CERTIFICATE DISTRIBUTION

A compilation of entire work in the form of report has to be prepared by every student. All the results and finding has to be added in it along with the analysis (If any)  by the students under constant guidance of supervisor

ALL TRAINING MODULES

MODULES FOR 7 DAYS

APPLICATION OF MICROBIOLOGY

  • Grams staining of Bacteria

  • Lactophenol cotton blue staining of Fungus

  • IMViC Test

  • Growth Kinetics of Microbes

  • Antibiotic sensitivity test

  • Anti-fungal Test

  • Minimum Inhibitory concentration (MIC)

  • Minimum Bactericidal concentration (MBC)

  • Minimum Fungicidal Test (MFC)

  • Antagonistic Activity​

Duration: 7 Days​        

Fee: 4000/- (Reg. fee 500 + Training Fee 3500)

APPLICATION OF
MOLECULAR BIOLOGY

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • Quantification of DNA  (Quality Check)

  • PCR Amplification of Gene of Interest & its Electrophoresis

  • RNA isolation from animal tissue

  • Formaldehyde Gel Electrophoresis

  • Quantification of RNA (Quality Check)

  • cDNA Synthesis (by reverse Transcriptase)

Duration: 7 Days​        

Fee: 4000/- (Reg. fee 500 + Training Fee 3500)

APPLICATION OF ENZYME TECHNOLOGY

  • Production of Enzyme from Bacteria

  • Enzyme Quantification by Lowry’s Method

  • Determination of Enzyme activity

  • Calculation of IC-50 Value

  • SDS-PAGE (Vertical Electrophoresis)

  • Enzyme Kinetics

  • Zymogram

Duration: 7 Days​        

Fee: 4000/- (Reg. fee 500 + Training Fee 3500)

MODULES FOR 15 DAYS

APPLICATION OF MICROBIOLOGY AND MOLECULAR BIOLOGY

Microbiology

  • Grams staining of Bacteria

  • Lactophenol cotton blue staining of Fungus

  • IMViC Test

  • Growth Kinetics of Microbes

  • Antibiotic sensitivity test

  • Anti-fungal Test

  • Minimum Inhibitory concentration (MIC)

  • Minimum Bactericidal concentration (MBC)

  • Minimum Fungicidal Test (MFC)

  • Antagonistic Activity​

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Molecular Biology

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • Quantification of DNA  (Quality Check)

  • PCR Amplification of Gene of Interest & its Electrophoresis

  • RNA isolation from animal tissue

  • Formaldehyde Gel Electrophoresis

  • Quantification of RNA (Quality Check)

  • cDNA Synthesis (by reverse Transcriptase)

Duration: 15 Days​        

Fee: 5500/- (Reg. fee 500 + Training Fee 5000)

APPLICATION OF MOLECULAR
BIOLOGY & BIOCHEMISTRY

Molecular BIology

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • Quantification of DNA  (Quality Check)

  • PCR Amplification of Gene of Interest & its Electrophoresis

  • RNA isolation from animal tissue

  • Formaldehyde Gel Electrophoresis

  • Quantification of RNA (Quality Check)

  • cDNA Synthesis (by reverse Transcriptase)

 

Biochemistry​

  • Secondary Metabolites (by Soxhlet)

  • Antioxidant Assay (by DPPH)

  • Superoxide Dismutase

  • Scan by UV-Vis Spectrophotometer

  • Total phenolic content

  • Total flavonoid content

  • Sugar Estimation by DNS Assay

Duration: 15 Days​        

Fee: 5500/- (Reg. fee 500 + Training Fee 5000)

APPLICATION OF  MOLECULAR 
BIOLOGY & ENZYME TECHNOLOGY

Molecular Biology

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • Quantification of DNA  (Quality Check)

  • PCR Amplification of Gene of Interest & its Electrophoresis

  • RNA isolation from animal tissue

  • Formaldehyde Gel Electrophoresis

  • Quantification of RNA (Quality Check)

  • cDNA Synthesis (by reverse Transcriptase)

 

Enzyme Technology​

  • Production of Enzyme from Bacteria

  • Enzyme Quantification by Lowry’s Method

  • Determination of Enzyme activity

  • Calculation of IC-50 Value

  • SDS-PAGE (Vertical Electrophoresis)

  • Enzyme Kinetics

  • Zymogram

Duration: 15 Days​        

Fee: 5500/- (Reg. fee 500 + Training Fee 5000)

Timing: 4-5hr (in between 09am to 5pm)

Working Days: 5 Days (Mon – Fri)
No of Seats: 10/Batch (First Come First Serve Basis)

MODULE FOR 30 DAYS

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APPLICATION OF BIOTECHNOLOGY

Microbiology

  • Grams staining of Bacteria

  • Lactophenol cotton blue staining of Fungus

  • IMViC Test

  • Growth Kinetics of Microbes

  • Antibiotic sensitivity test

  • Anti-fungal Test

  • Minimum Inhibitory concentration (MIC)

  • Minimum Bactericidal concentration (MBC)

  • Minimum Fungicidal Test (MFC)

  • Antagonistic Activity​

 

Molecular Biology

  • Genomic DNA Isolation from Blood

  • Agarose Gel Electrophoresis

  • Quantification of DNA  (Quality Check)

  • PCR Amplification of Gene of Interest & its Electrophoresis

  • RNA isolation from animal tissue

  • Formaldehyde Gel Electrophoresis

  • Quantification of RNA (Quality Check)

  • cDNA Synthesis (by reverse Transcriptase)

 

Enzyme Technology​

  • Production of Enzyme from Bacteria

  • Enzyme Quantification by Lowry’s Method

  • Determination of Enzyme activity

  • Calculation of IC-50 Value

  • SDS-PAGE (Vertical Electrophoresis)

  • Enzyme Kinetics

  • Zymogram

 

Biochemistry

  • Extraction (by Soxhlet)

  • Antioxidant Assay (by DPPH)

  • Superoxide Dismutase

  • Scan by UV-Vis Spectrophotometer

  • Total phenolic content

  • Total flavonoid content

  • Sugar Estimation by DNS Assay

Duration: 30 Days​        

Fee: 9500/- (Reg. fee 500 + Training Fee 9000)

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TECHNIQUES OF BIOTECHNOLOGY

Microbiology

  • Media Preparation & Sterilization

  • Serial Dilution

  • Spread Plate Method

  • Streak Plate Method

  • Culture Broth Preparation

  • Gram’s staining

  • Acid Fast staining

  • Antibiotic sensitivity test

 

Molecular Biology

  • Plasmid isolation from bacteria

  • Agarose gel Electrophoresis

  • DNA isolation from plant leaves

  • Agarose gel Electrophoresis

  • RNA isolation from plant leaves

  • Formaldehyde Gel Electrophoresis

  • Quantification of Nucleotides

  • PCR Amplification & its Electrophoresis

 

Enzyme Technology​

  • Enzyme & Fermentation Technology

  • Media Preparation and sterilization

  • Screening of enzyme producing bacteria

  • Enzyme production from submerged fermentation

  • Enzyme production from plant tissue

  • Precipitation of enzyme

  • Estimation by Bradford’s method

  • Enzyme Kinetics

  • Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).

 

Biochemistry

  • Isolation of Protein from different sources.

  • Protein estimation by Lowry’s Method

  • Separation of amino acids by Thin Layer Chromatography.

  • Separation of compounds by Paper Chromatography.

  • Purification by Column Chromatography.

  • Estimation of Sugar by DNS Assay.

  • Estimation of Cholesterol.

  • Estimation of Amino Acid.

Duration: 30 Days​        

Fee: 7500/- (Reg. fee 500 + Training Fee 7000)

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SUPERVISORS

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Dr. Madhulika Singh

Area of Specialisation: Molecular Biology, Microbiology & Bioinformatics

 

Research Experience: 10+ years

Trainees Undergone: 2500+

COMPULSORY REQUIREMENT

 

  1. Basic Handling on Instruments/Laboratory (For Application Module)

  2. A laptop with Basic configuration (For report Prepration)

  3. A Lab coat

BATCHES IN 2023

Mar 15, April 01 & 17, May 01 & 15, June 01, 12, 19 & 26, July 01, 10 & 20

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Register in any Batch

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Till 31 March 2023 and get 10% OFF on training fee 

or

Till 30 April 2023 and get 5% OFF on training fee

HOW TO REGISTER

Online Registration: 

Click the blow given button to register online. Fill the form and submit. Pay registration fee Rs. 500/- by any given Mode. Confirmation letter will be generated and sent by email. Training fee can be paid after receiving of confirmation letter till the date of Joining.

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Offline Registration: 

Download MS Word format of Registration form.

Send the duly filled registration form and screenshot of payment by Email to training@cytogene.in or

Whatsapp to 77-0309-2222

Click to download registration form

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Mode of Payment: https://www.cytogene.in/mode-of-payment

CONTACT PERSON

Er. Sujeet Kumar Singh

Director

CytoGene Research & Development

Lucknow - 226021

+91-77-0309-2222 , +91-77-0309-3333

training@cytogene.in 

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